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KMID : 0364820080440020098
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Korean Journal of Microbiology
2008 Volume.44 No. 2 p.98 ~ p.104
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Genetic Analysis of Fission Yeast rsm1 Which is Involved in mRNA Export
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Kang Suky
Yoon Jin-Ho
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Abstract
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We constructed the null mutants of fission yeast Schizosaccharomyces pombe rsm1 gene that is thought to be
involved in mRNA export. Though rsm1 gene is not essential for growth, the null mutant strain constructed by
replacing the rsm1-coding region with an kanr gene showed growth retardation and mRNA export defects compared
to wild type strain. We constructed double mutants which harbor rsm1 null allele and mutant allele of
genes involved in mRNA export. The mex67 or npp106 null allele, when combined with rsm1 null allele,
showed an additive effect on growth retardation and mRNA export defects. On the other hand, the thp1 null
allele restored the defects of growth and mRNA export of rsm1 null mutant. These results suggest that rsm1
plays a role in mRNA export from the nucleus.
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KEYWORD
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genetic analysis, mRNA export, rsm1, Schizosaccharomyces pombe
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